A Chemical Biology Solution to Problems with Studying Biologically Important but Unstable 9-O-Acetyl Sialic Acids
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چکیده
منابع مشابه
Sialic acids in human gastric aspirates: detection of 9-O-lactyl- and 9-O-acetyl-N-acetylneuraminic acids and a decrease in total sialic acid concentration with age.
1. The total sialic acid content of human gastric aspirates was measured using a colorimetric assay. Care was taken to optimize the assay and to eliminate interference. 2. The sialic acid content of gastric aspirates collected under resting conditions from 77 patients with non-ulcer dyspepsia was found to decrease with age from > 100 micrograms/ml at 25 years and younger to < 20 micrograms/ml a...
متن کامل9-O-Acetylation of sialic acids is catalysed by CASD1 via a covalent acetyl-enzyme intermediate
Sialic acids, terminal sugars of glycoproteins and glycolipids, play important roles in development, cellular recognition processes and host-pathogen interactions. A common modification of sialic acids is 9-O-acetylation, which has been implicated in sialoglycan recognition, ganglioside biology, and the survival and drug resistance of acute lymphoblastic leukaemia cells. Despite many functional...
متن کاملO-acetylation and de-O-acetylation of sialic acids. Purification, characterization, and properties of a glycosylated rat liver esterase specific for 9-O-acetylated sialic acids.
We have previously described the preparation and use of 9-O-[acetyl-3H]acetyl-N-acetylneuraminic acid to identify sialic acid O-acetylesterases in tissues and cells (Higa, H. H., Diaz, S., and Varki, A. (1987) Biochem. Biophys. Res. Commun. 144, 1099-1108). All tissues of the adult rat showed these activities, with the exception of plasma. Rat liver contained two major sialic acid esterases: a ...
متن کاملSelective inactivation of influenza C esterase: a probe for detecting 9-O-acetylated sialic acids.
The influenza C virus (INF-C) hemagglutinin recognizes 9-O-acetyl-N-acetylneuraminic acid. The same protein contains the receptor-destroying enzyme (RDE), which is a 9-O-acetyl-esterase. The RDE was inactivated by the serine esterase inhibitor di-isopropyl fluorophosphate (DFP). [3H]DFP-labeling localized the active site to the heavy chain of the glycoprotein. DFP did not alter the hemagglutina...
متن کاملThe release and purification of sialic acids from glycoconjugates: methods to minimize the loss and migration of O-acetyl groups.
The sialic acids can have O-acetyl esters at the 4, 7, 8, and 9 positions. Most methods for the detailed study of such molecules require their release from glycosidic linkage, followed by extensive purification. The currently used methods for release and purification of sialic acids allow a reasonable qualitative analysis of the diversity of sialic acids from a given biological source. However,...
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ژورنال
عنوان ژورنال: ACS Chemical Biology
سال: 2016
ISSN: 1554-8929,1554-8937
DOI: 10.1021/acschembio.6b00928